P4 FPA
A quantitative milk progesterone assay
Detect pathological conditions
P4 FPA
A quantitative milk progesterone assay
Detect pathological conditions
What is the
P4 FPA?
The P4 FPA is a quantitative diagnostic test that uses Fluorescence Polarization (FP) technology to detect progesterone levels in milk samples of cattle. It is a simple, fast, and accurate test.
Unlike other technologies, the P4 FPA is a homogenous test conducted in the presence of alcohol. This makes the test easy to perform, consistent, and accurate.
THE P4 FPA IS A FARM OR LABORATORY ASSAY
- Detection of proestrus (open cows) as early as day 18 of the cycle
- Detection of irregular estrous cycles
- Detection of pathological conditions of the corpus luteum and follicle (insufficiency, luteal or follicular cysts, non-cycling, persistent corpus luteum, and others)
- Supplemental method for synchronization
THE P4 FPA IS A FARM OR LABORATORY ASSAY
- Detection of proestrus (open cows) as early as day 18 of the cycle
- Detection of irregular estrous cycles
- Detection of pathological conditions of the corpus luteum and follicle (insufficiency, luteal or follicular cysts, non-cycling, persistent corpus luteum, and others)
- Supplemental method for synchronization
Performance
In the graph below, the X-axis represents days post-artificial insemination. Test results on the Y-axis are provided in ΔmP units. The cutoff is 35 ΔmP. Cows with results lower than 35 ΔmP have a low progesterone level and are considered open. Note that it is easy to identify opened cows as early as day 18. You can also see that some of the pregnant cows started to show signs of heat. The P4 FPA shows 100% specificity. No pregnant cows were identified as open.
The data in this report unambiguously demonstrates the strong analytical and diagnostic performance of the milk P4 FPA developed by Ellie LLC. A specificity of 100% is critical in preventing potentially dangerous repeated insemination of already-pregnant cows. Pre-estrous cows and cows with silent estrus were also consistently identified. In tightly controlled situations (e.g., the inclusion of a synchronization protocol), the assay demonstrated high sensitivity and specificity. We expect the assay’s performance to facilitate a significant financial gain via the early detection of opened cows.
Please click on the link and read our Ellie Technical Note (ETN).
Ordering
Information
Cattle Milk Progesterone Test Kit, FPA
| Trade name | P4 FPA |
|---|---|
| Technology | Flourescence Polarization Technology |
| Analyte | Progesterone |
| Species | Bovine |
| Samples | Milk |
| Detection type | Quantitative |
| Ordering info | P1001-250 (250 tests kit) P1001-1000 (1000 tests kit) |
A quantitative assay that detects progesterone (P4) in milk samples of cattle. The P4 FPA is run completely in solution without binding to plastic or paper, without washing steps, and in the presence of alcohol, which helps dissolve P4 out of milk. The test requires the use of FP readers and can be run cow-side or in simple laboratories. The P4 FPA is based on a patent-pending technology developed by Ellie LLC.